goat anti mouse relmα himf fizz1 antibody  (R&D Systems)

Journal: PLoS ONE

Article Title: Riociguat prevents hyperoxia-induced lung injury and pulmonary hypertension in neonatal rats without effects on long bone growth

doi: 10.1371/journal.pone.0199927

Figure Lengend Snippet: ( A ) Immunostaining for Mac-3, a macrophage marker. ( B ) The alveolar airspace macrophage population was increased by hyperoxia exposure as compared to normoxia. Administration of riociguat decreased macrophage count during hyperoxia. *** P < 0.001 compared with normoxia; ++ P < 0.01 compared with hyperoxia + placebo (n = 6/group). ( C ) Immunostaining for the M1 marker, inducible nitric oxide synthase (iNOS), and M2 markers, chitinase 3-like 3 (Ym1) and resistin-like molecule alpha (RELM-α) showed that in hyperoxia plus placebo lungs, both M1 and M2 polarized macrophages were detected. But, treatment with riociguat decreased only M1 macrophages in hyperoxia-exposed lungs. ( D ) Representative Western blots for NLRP-1, active caspase-1 and active IL-1β. Administration of riociguat decreased hyperoxia-induced lung expression of ( E ) NLRP-1 (*** P < 0.001 compared with normoxia; + P < 0.05 compared with hyperoxia + placebo), ( F ) active caspase-1 (*** P < 0.001 compared with normoxia; ++ P < 0.05 compared with hyperoxia + placebo), and ( G ) active IL-1β (* P < 0.05 compared with normoxia; +++ P < 0.001 compared with hyperoxia + placebo). RA: room air, normoxia; O 2 : hyperoxia; PL: placebo; Rio: riociguat.

Article Snippet: The following antibodies were used for immunostaining, double immunofluorescence staining and Western blot analyses: a rabbit anti-vonWillebrand factor (vWF) antibody from Dako (Carpinteria, CA); a mouse anti- α-smooth muscle actin (α-SMA) from Sigma (Saint Louis, MI); a rat anti-Mac3 antibody from BD Biosciences (San Jose, CA); rabbit anti-Ki67, anti-inducible nitric oxide synthase (iNOS), anti-chitinase 3-like 3 (Ym1), and anti-NLR family pyrin domain containing 1 (NLRP-1) antibodies from Abcam (Cambridge, MA); rabbit anti-caspase-1 and anti-connective tissue growth factor (CTGF) antibodies, and a goat anti-resistin-like molecule alpha (RELM-α) antibody from Santa Cruz (Dallas, TX)); a goat anti-IL-1β antibody from R&D System (Minneapolis, MN).

Techniques: Immunostaining, Marker, Western Blot, Expressing

Journal: Stem Cells and Development

Article Title: Resistin-Like Molecule ? Stimulates Proliferation of Mesenchymal Stem Cells While Maintaining Their Multipotency

doi: 10.1089/scd.2012.0192

Figure Lengend Snippet: Effect of RELMα on osteogenic and myogenic differentiation of MSCs. (A) Osteogenic differentiation was stimulated for 3 weeks with osteogenic medium, cells were stained with Alizarin Red for calcium phosphate deposits. The staining was quantified using ImageJ softwate (*P=0.2). (B) Western blot shows the expression of osteopontin. (C) Myogenic differentiation was induced with 10 ng/mL transforming growth factor β1 in growth medium for 5 days. Western blot shows the expression of smooth muscle actin and fibronectin as markers of myofibroblasts.

Article Snippet: The following antibodies were used: phospho-p38 MAPK (Thr180/Tyr182) mouse mAb, phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit mAb, Egr-1 rabbit mAb, phospho-Akt, phospho-p65 (Ser536) rabbit mAb, adiponectin rabbit mAb, and β-Tubulin rabbit mAb (Cell Signaling Technology); β-actin mouse mAb clone AC-15 and GAPDH mouse mAb (Sigma-Aldrich); RELMα goat antibody (R & D Systems); osteopontin mouse mAb (Abcam), fibronectin mouse mAb, fluorescein isothiocyanate (FITC)-labeled rat anti-mouse Ly-6A/E (Sca-1) Ab, and FITC-labeled rat anti-mouse CD45 Ab (BD Biosciences).

Techniques: Staining, Western Blot, Expressing